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mouse anti synapsin antibody  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank mouse anti synapsin antibody
    Mouse Anti Synapsin Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+synapsin/anti-Synapsin/pm42128921-96-8-17
    Average 95 stars, based on 47 article reviews
    mouse anti synapsin antibody - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: Mitochondrial Complex I and ROS control neuromuscular function through opposing pre- and postsynaptic mechanisms.
    Article Snippet: .. Monoclonal antibodies: anti-Dlg (4F3), anti-DGluRIIA (8B4D2), anti-CSP (ab49), anti-Synapsin (3C11), anti-Futsch (22C10), anti-Bruchpilot (nC82), and anti-α-Spectrin (3A9) were obtained from the Developmental Studies Hybridoma Bank (University of Iowa, IA, USA) and were used at 1:30 dilution. .. Rabbit anti-GFP (Abcam) was used at 1:200 dilutions.

    Article Title: Mitochondrial Complex I and ROS control neuromuscular function through opposing pre- and postsynaptic mechanisms
    Article Snippet: .. Monoclonal antibodies: anti-Dlg (4F3), anti-DGluRIIA (8B4D2), anti-CSP (ab49), anti-Synapsin (3C11), anti-Futsch (22C10), anti-Bruchpilot (nC82), and anti-α-Spectrin (3A9) were obtained from the Developmental Studies Hybridoma Bank (University of Iowa, IA, USA) and were used at 1:30 dilution. .. Rabbit anti-GFP (Abcam) was used at 1:200 dilutions.

    Incubation:

    Article Title: NR1I3 modulates Wnt signaling to promote anterior–posterior axis patterning
    Article Snippet: .. Then, the samples were blocked with 10% goat serum in PBST for 2 h RT and incubated with primary anti-synapsin (1:100; Developmental Studies Hybridoma Bank) or anti-H3P antibodies (1:250; Millipore, 05-817R) overnight at 4 °C. ..

    Article Title: NR1I3 modulates Wnt signaling to promote anterior-posterior axis patterning.
    Article Snippet: .. Then, the samples were blocked with 10% goat serum in PBST for 2 h RT and incubated with primary anti-synapsin (1:100; Developmental Studies Hybridoma Bank) or anti-H3P antibodies (1:250; Millipore, 05-817R) overnight at 4 °C. ..

    Article Title: Excessive activation of the RAS/MAPK pathway triggers adult-onset motor axonal degeneration
    Article Snippet: Third instar larvae were dissected in HL3 buffer (70 mM NaCl, 5mM KCl, 1.5 mM CaCl 2 , 20mM MgCl 2 , 10 mM NaHCO 3 , 5 mM trehalose, 115 mM sucrose, and 5 mM Hepes 5 (pH 7.2)), and fixed in Bouin’s (HT10132, Sigma-Aldrich) for 3 min (for anti-Dlg1 and anti-Brp) or fixed in 4% FA (52549, Sigma-Aldrich) in PBS, pH 7.2, for 30 min (for anti-Syn and anti-Rop). .. Three 15 min washes were performed in PBT (PBS pH7.2, 0.2% Triton-X-100 (T8787, Sigma-Aldrich)), before blocking in 10% goat serum (16210064, Gibco) in PBT for 1 h. Incubation with primary antibody – either anti-Dlg1 (mouse monoclonal 4F3; DSHB; 1:200), anti-Brp (mouse monoclonal nc82; DSHB; 1:200), anti-Synapsin (mouse monoclonal 3C11; DSHB; 1:200) or anti-Rop (mouse monoclonal 4F8; DSHB; 1:100) – was performed in blocking solution O/N at 4 °C. .. Third instar larvae were dissected in HL3 buffer (70 mM NaCl, 5mM KCl, 1.5 mM CaCl 2 , 20mM MgCl 2 , 10 mM NaHCO 3 , 5 mM trehalose, 115 mM sucrose, and 5 mM Hepes 5 (pH 7.2)), and fixed in Bouin’s (HT10132, Sigma-Aldrich) for 3 min (for anti-Dlg1 and anti-Brp) or fixed in 4% FA (52549, Sigma-Aldrich) in PBS, pH 7.2, for 30 min (for anti-Syn and anti-Rop).

    Staining:

    Article Title: Ecological divergence and post-eclosion brain development shape visual performance during Heliconius speciation
    Article Snippet: .. Brains were fixed in zinc-formaldehyde, stained using anti-synapsin (DSHB, RRID: AB_2315424), and imaged with confocal microscopy following previous studies ( , ). ..

    Confocal Microscopy:

    Article Title: Ecological divergence and post-eclosion brain development shape visual performance during Heliconius speciation
    Article Snippet: .. Brains were fixed in zinc-formaldehyde, stained using anti-synapsin (DSHB, RRID: AB_2315424), and imaged with confocal microscopy following previous studies ( , ). ..

    Blocking Assay:

    Article Title: Excessive activation of the RAS/MAPK pathway triggers adult-onset motor axonal degeneration
    Article Snippet: Third instar larvae were dissected in HL3 buffer (70 mM NaCl, 5mM KCl, 1.5 mM CaCl 2 , 20mM MgCl 2 , 10 mM NaHCO 3 , 5 mM trehalose, 115 mM sucrose, and 5 mM Hepes 5 (pH 7.2)), and fixed in Bouin’s (HT10132, Sigma-Aldrich) for 3 min (for anti-Dlg1 and anti-Brp) or fixed in 4% FA (52549, Sigma-Aldrich) in PBS, pH 7.2, for 30 min (for anti-Syn and anti-Rop). .. Three 15 min washes were performed in PBT (PBS pH7.2, 0.2% Triton-X-100 (T8787, Sigma-Aldrich)), before blocking in 10% goat serum (16210064, Gibco) in PBT for 1 h. Incubation with primary antibody – either anti-Dlg1 (mouse monoclonal 4F3; DSHB; 1:200), anti-Brp (mouse monoclonal nc82; DSHB; 1:200), anti-Synapsin (mouse monoclonal 3C11; DSHB; 1:200) or anti-Rop (mouse monoclonal 4F8; DSHB; 1:100) – was performed in blocking solution O/N at 4 °C. .. Third instar larvae were dissected in HL3 buffer (70 mM NaCl, 5mM KCl, 1.5 mM CaCl 2 , 20mM MgCl 2 , 10 mM NaHCO 3 , 5 mM trehalose, 115 mM sucrose, and 5 mM Hepes 5 (pH 7.2)), and fixed in Bouin’s (HT10132, Sigma-Aldrich) for 3 min (for anti-Dlg1 and anti-Brp) or fixed in 4% FA (52549, Sigma-Aldrich) in PBS, pH 7.2, for 30 min (for anti-Syn and anti-Rop).



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